anti wave2 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti wave2
Anti Wave2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave2/pm41698632-109-21-22
Average 86 stars, based on 1 article reviews
Anti Wave2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wave2/pm41698632-109-21-22
Average 86 stars, based on 1 article reviews
anti wave2 - by Bioz Stars,
2026-10
86/100 stars
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Western Blot:Article Title: Hem1 is essential for ruffled border formation in osteoclasts and efficient bone resorption. Article Snippet: After stimulation, whole cell lysates of osteoclasts were lysed in NP-40 buffer containing (150 mM sodium chloride, 1% NP-40, 50 mM Tris pH8) including protease and phosphatase inhibitor cocktail (#11,873,580,001, #P5726, #P004; Sigma Aldrich). .. Specific antibodies against the following proteins were detected by immunoblotting: cathepsin K (abcam, ab 19,027, 1:1000), integrin alpha-v (abcam, ab179475,1:1000), integrin beta-3 (Cell Signaling #4702,1:1000), DC-STAMP (sc-98769, 1:200), NFATc1 (sc-7294; both from Santa Cruz), Hem1 (Novus Bio, NBP2-13,643, 1:1000), WASP (Santa Cruz, sc-13139, 1:1000) and Article Title: Neurodevelopmental disorder-associated CYFIP2 regulates membraneless organelles and eIF2α phosphorylation via protein interactors and actin cytoskeleton. Article Snippet: De novo variants in the Cytoplasmic FMR1-interacting protein 2 (CYFIP2) have been repeatedly associated with neurodevelopmental disorders and epilepsy, underscoring its critical role in brain development and function.. While CYFIP2’s role in regulating actin polymerization as part of the WAVE regulatory complex (WRC) is well-established, its additional molecular functions remain relatively unexplored.. In this study, we performed unbiased quantitative proteomic analysis, revealing 278 differentially expressed proteins (DEPs) in the forebrain of Cyfip2 knock-out embryonic mice compared to wild-type mice. Article Title: Hem1 is essential for ruffled border formation in osteoclasts and efficient bone resorption Article Snippet: After stimulation, whole cell lysates of osteoclasts were lysed in NP-40 buffer containing (150 mM sodium chloride, 1% NP-40, 50 mM Tris pH8) including protease and phosphatase inhibitor cocktail (#11,873,580,001, #P5726, #P004; Sigma Aldrich). .. Specific antibodies against the following proteins were detected by immunoblotting: cathepsin K (abcam, ab 19,027, 1:1000), integrin alpha-v (abcam, ab179475,1:1000), integrin beta-3 (Cell Signaling #4702,1:1000), DC-STAMP (sc-98769, 1:200), NFATc1 (sc-7294; both from Santa Cruz), Hem1 (Novus Bio, NBP2-13,643, 1:1000), WASP (Santa Cruz, sc-13139, 1:1000) and Incubation:Article Title: Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia Article Snippet: The protein from the gel was then transferred onto a nitrocellulose membrane (#GE10600003, Amersham Biosciences) using a semi-dry transfer approach. .. After incubation with 5% non-fat milk in TBS-T (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was washed twice with TBS-T and incubated with primary antibodies against Rac1 (1:1000, #2465 s, Cell Signaling Technology), phospho Rac1 (1:500, #2461 S, Cell Signaling Technology), PAK1 (1:500, 51137-1-AP, Protein Tech), phospho PAK1 (1:1000, #2601, Cell Signaling Technology), Article Title: Mesenchymal stem cell-derived exosomes rescue stress-dependent morphological deficits in primary hippocampal neurons via Wnt5a/β-catenin/WAVE2 pathway. Article Snippet: Objectives: Increasing evidence indicates that chronic stress impairs dendritic branching and synaptic plasticity in hippocampal neurons.. Mesenchymal stem cell-derived exosomes (MSC-Exos) are crucial for tissue injury repair, facilitating cell communication and transporting bioactive cargo like lipids, proteins, and nucleic acids.. Herein, we investigate the therapeutic effects and molecular mechanism of MSC-Exos against stress-dependent morphological deficits in primary hippocampal neurons. Membrane:Article Title: Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia Article Snippet: The protein from the gel was then transferred onto a nitrocellulose membrane (#GE10600003, Amersham Biosciences) using a semi-dry transfer approach. .. After incubation with 5% non-fat milk in TBS-T (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was washed twice with TBS-T and incubated with primary antibodies against Rac1 (1:1000, #2465 s, Cell Signaling Technology), phospho Rac1 (1:500, #2461 S, Cell Signaling Technology), PAK1 (1:500, 51137-1-AP, Protein Tech), phospho PAK1 (1:1000, #2601, Cell Signaling Technology), Article Title: The WAVE complex associates with sites of saddle membrane curvature Article Snippet: .. For primary antibodies IRSp53 (rabbit; 1:100; Abcam; #ab37542), β-actin (mouse; 1:1,000; Cell Signaling Technology; #3700), SDS Page:Article Title: The WAVE complex associates with sites of saddle membrane curvature Article Snippet: .. For primary antibodies IRSp53 (rabbit; 1:100; Abcam; #ab37542), β-actin (mouse; 1:1,000; Cell Signaling Technology; #3700), |
